| Field | Specification |
|---|---|
| Target | |
| Alternative names | Dimethylaniline monooxygenase [N-oxide-forming] 3;1.14.13.8;Dimethylaniline oxidase 3;FMO II;FMO form 2;Hepatic flavin-containing monooxygenase 3;FMO 3;Trimethylamine monooxygenase;1.14.13.148;FMO3; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human FMO3, different from the related mouse sequence by eight amino acids, and from the related rat sequence by six amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
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| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
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Product Overview
This rabbit polyclonal antibody detects flavin-containing monooxygenase 3 (gene FMO3) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 60.0 kDa, and the supplier reports an observed band at about 60 kDa.
Flavin-containing monooxygenase 3 (FMO3) is the main FMO in adult liver and oxygenates many nitrogen- and sulfur-containing drugs and dietary compounds. It converts trimethylamine from gut bacteria into trimethylamine N-oxide (TMAO), and loss-of-function mutations cause trimethylaminuria. FMO3 is studied in drug metabolism and in TMAO and cardiometabolic research.
| Target | Flavin-containing monooxygenase 3 (gene FMO3; UniProt P31513, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 60.0 kDa |
| Observed MW | 60 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): human hepatocellular carcinoma tumor tissue, human hepatocellular carcinoma paracancerous tissue, rat liver tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human FMO3. It differs from the mouse sequence at eight residues and from the rat sequence at six residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places flavin-containing monooxygenase 3 in the microsome membrane and endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
