| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fibronectin type III domain-containing protein 5; Fibronectin type III repeat-containing protein 2; FNDC5; FRCP2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human FNDC5 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18F66 is a rabbit monoclonal antibody that recognizes fibronectin type III domain-containing protein 5 (gene FNDC5) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 23.7 kDa, and the supplier reports an observed band at about 24 kDa.
FNDC5 is a membrane protein expressed mainly in skeletal muscle; its extracellular portion is cleaved to release the hormone-like peptide irisin. Irisin has been reported to be increased by exercise and to promote browning of white fat, although parts of this model are still debated. FNDC5/irisin is studied in exercise physiology and metabolism.
| Target | Fibronectin type III domain-containing protein 5 (gene FNDC5; UniProt Q8NAU1, human) |
|---|---|
| Clone | 18F66 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 23.7 kDa |
| Observed MW | 24 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): rat muscle cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human FNDC5.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places fibronectin type III domain-containing protein 5 in the peroxisome membrane. Tissue expression noted by UniProt (human): Widely expressed, with highest levels in heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jingjing Yang et al. (2024) Irisin alleviates the pyroptosis of β cells in T2DM by inhibiting NLRP3 inflammasome through regulating miR-19b-3p/SOCS3/STAT3 axis mediated autophagy. IUBMB Life. 10.1002/iub.2907
