| Field | Specification |
|---|---|
| Target | |
| Alternative names | Hepatocyte nuclear factor 3-beta; HNF-3-beta; HNF-3B; Forkhead box protein A2; Transcription factor 3B; TCF-3B; FOXA2; HNF3B; TCF3B |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human FOXA2 recombinant protein (Position: Q139-Q446). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects hepatocyte nuclear factor 3-beta (gene FOXA2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 48.3 kDa, and the supplier reports an observed band at about 48 kDa.
FOXA2 (HNF-3β) is a forkhead transcription factor and pioneer factor that opens chromatin for other regulators. It is essential for forming the node, notochord and endoderm in early development and for liver, pancreas and lung development, and it regulates metabolic genes in the adult liver. FOXA2 is a common endoderm marker in stem cell research.
| Target | Hepatocyte nuclear factor 3-beta (gene FOXA2; UniProt Q9Y261, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 48.3 kDa |
| Observed MW | 48 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HepG2 cells, human PC-3 cells, human A549 cells, human HeLa cells, human Caco-2 cells, rat pancreas tissue, mouse pancreas tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human colonic adenocarcinoma tissue, human gastric cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): PC-3 cells.
Samples with a confirmed signal (flow cytometry): 293T cells.
Immunogen
Recombinant human FOXA2 fragment (Gln139–Gln446), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places hepatocyte nuclear factor 3-beta in the nucleus and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jiekun Sun et al. (2026) Decellularized scaffolds of porcine uterus reconstruct the endometrial microenvironment to promote uterine epithelial cell differentiation and organoid formation. Journal of Materials Chemistry B. 10.1039/D6TB00637J
