| Field | Specification |
|---|---|
| Target | |
| Alternative names | Forkhead box protein F1; Forkhead-related activator 1; FREAC-1; Forkhead-related protein FKHL5; Forkhead-related transcription factor 1; FOXF1; FKHL5; FREAC1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human FOXF1 recombinant protein (Position: R44-M379). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects forkhead box protein F1 (gene FOXF1) in human, mouse and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 40.1 kDa, and the supplier reports an observed band at about 38–40 kDa.
FOXF1 is a forkhead transcription factor expressed in the mesenchyme of the lung and gut. It is needed for normal lung, gut and blood vessel development and regulates lung-specific genes. FOXF1 mutations cause alveolar capillary dysplasia with misalignment of pulmonary veins.
| Target | Forkhead box protein F1 (gene FOXF1; UniProt Q12946, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 40.1 kDa |
| Observed MW | 38–40 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human A549 cells, human HeLa cells, human PC-3 cells, human U2OS cells, mouse liver tissue, mouse SP20 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human oesophagus squama cancer tissue, human rectal cancer tissue, rat lung tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): Jurkat cells.
Immunogen
Recombinant human FOXF1 fragment (Arg44–Met379), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places forkhead box protein F1 in the nucleus. Tissue expression noted by UniProt (human): Expressed in lung and placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xue Wang et al. (2025) FOXF1 and SHH participate in the regulation of iron signaling in pulmonary fibrosis. Redox Biology. 10.1016/j.redox.2025.103893
Xu Lingling et al. (2022) LINC00022 acts as an oncogene in colorectal cancer progression via sponging miR-375-3p to regulate FOXF1 expression. BMC Cancer. 10.1186/s12885-022-09566-5
