| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Forkhead box protein F1; Forkhead-related activator 1; FREAC-1; Forkhead-related protein FKHL5; Forkhead-related transcription factor 1; FOXF1; FKHL5; FREAC1 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human FOXF1 recombinant protein (Position: R44-M379). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-FOXF1 Picoband® Antibody is an antibody for FOXF1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: FOXF1 (forkhead box F1); UniProt: Q12946
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 38-40 kDa, calculated 75187 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-FOXF1 Picoband® Antibody catalog # A03563-1.
Biological background
Biological context: Probable transcription activator for a number of lung-specific genes.
Expression and localization notes: cellular localization: Nucleus., tissue context: Detected in adult brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, heart, colon, small intestine testis and thymus. Expression was strongest in heart and pancreas..
Common research applications
- Western blotting (WB): Compare FOXF1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of FOXF1 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify FOXF1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Forkhead box protein F1 is a protein that in humans is encoded by the FOXF1 gene. It is mapped to 16q24.1. This gene belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the regulation of pulmonary genes as well as embryonic development.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Nucleus.
- Tissue details: Detected in adult brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, heart, colon, small intestine testis and thymus. Expression was strongest in heart and pancreas.
- Research category: Cell Biology,Cell Type Markers,Developmental Biology,Reproduction,Tags & Cell Markers
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
