| Field | Specification |
|---|---|
| Target | |
| Alternative names | Forkhead box protein O1;Forkhead box protein O1A;Forkhead in rhabdomyosarcoma;FOXO1;FKHR, FOXO1A; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human FOXO1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18F20 is a rabbit monoclonal antibody that recognizes forkhead box protein O1 (gene FOXO1) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 69.7 kDa, and the supplier reports an observed band at about 70 kDa.
FOXO1 is a forkhead transcription factor and a main target of insulin and growth factor signaling. When AKT phosphorylates it, FOXO1 is kept out of the nucleus; otherwise it activates genes for gluconeogenesis, stress resistance, cell cycle arrest and apoptosis. FOXO1 is studied in metabolism, diabetes, immunity and cancer.
| Target | Forkhead box protein O1 (gene FOXO1; UniProt Q12778, human) |
|---|---|
| Clone | 18F20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 69.7 kDa |
| Observed MW | 70 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human FOXO1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places forkhead box protein O1 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed in umbilical endothelial cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Dong Peiyue et al. (2014) MiR-15a/b promote adipogenesis in porcine pre-adipocyte via repressing FoxO1. ACTA Biochimica et Biophysica Sinica. 10.1093/abbs/gmu043
