| Field | Specification |
|---|---|
| Target | |
| Alternative names | Forkhead box protein O1;Forkhead box protein O1A;Forkhead in rhabdomyosarcoma;FOXO1;FKHR, FOXO1A; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human FOXO1A recombinant protein (Position: N456-G655). Human FOXO1A shares 93% amino acid (aa) sequence identity with both mouse and rat FOXO1A. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets forkhead box protein O1 (gene FOXO1) in human and rat samples and is validated for Western blot. The predicted molecular weight is 69.7 kDa, and the supplier reports an observed band at about 70 kDa.
FOXO1 is a forkhead transcription factor and a main target of insulin and growth factor signaling. When AKT phosphorylates it, FOXO1 is kept out of the nucleus; otherwise it activates genes for gluconeogenesis, stress resistance, cell cycle arrest and apoptosis. FOXO1 is studied in metabolism, diabetes, immunity and cancer.
| Target | Forkhead box protein O1 (gene FOXO1; UniProt Q12778, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 69.7 kDa |
| Observed MW | 70 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Rat, Human) |
|---|
Samples with a confirmed band (WB): rat lung tissue, rat brain tissue, human COLO320 cells, human HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human FOXO1A fragment (Asn456–Gly655), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 93%.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places forkhead box protein O1 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed in umbilical endothelial cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Minan Li et al. (2021) Long noncoding RNA matrilineal expression gene 3 inhibits hepatocellular carcinoma progression by targeting microRNA-5195-3p and regulating the expression of forkhead box O1. Bioengineered. 10.1080/21655979.2021.2005986
Mohamed Nabil et al. (2020) Prolonged overnutrition with fructose or fat induces metabolic derangements in rats by disrupting the crosstalk between the hypothalamus and periphery: Possible amelioration with fenofibrate. European Journal of Pharmacology. 10.1016/j.ejphar.2020.173136
