| Field | Specification |
|---|---|
| Target | |
| Alternative names | Forkhead box protein O3;AF6q21 protein;Forkhead in rhabdomyosarcoma-like 1;FOXO3;FKHRL1, FOXO3A; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human FoxO3a |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HFD-6 is a rabbit monoclonal antibody that recognizes forkhead box protein O3 (gene FOXO3) in human, mouse and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 71.3 kDa, and the supplier reports an observed band at about 90 kDa.
FOXO3 is a forkhead transcription factor inhibited by AKT phosphorylation downstream of insulin and growth factor signaling. When active in the nucleus, it drives genes for autophagy, oxidative stress resistance, cell cycle arrest and apoptosis. FOXO3 is studied in stem cell maintenance, muscle atrophy, cancer and aging.
| Target | Forkhead box protein O3 (gene FOXO3; UniProt O43524, human) |
|---|---|
| Clone | HFD-6 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 71.3 kDa |
| Observed MW | 90 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human foxO3a.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places forkhead box protein O3 in the cytoplasm, nucleus, mitochondrion matrix and mitochondrion outer membrane. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Shouchao Xu et al. (2023) Calycosin alleviates cerebral ischemia/reperfusion injury by repressing autophagy via STAT3/FOXO3a signaling pathway. Phytomedicine. 10.1016/j.phymed.2023.154845
Jiang‑Shui Yuan et al. (2020) Holliday junction‑recognition protein modulates apoptosis, cell cycle arrest and reactive oxygen species stress in human renal cell carcinoma. Oncology Reports. 10.3892/or.2020.7656
Meigui Zhang et al. (2023) (2E)-1-(2,4,6-Trimethoxyphenyl)-3-(4-chlorophenyl)prop-2-en-1-one, a Chalcone Derivative, Promotes Apoptosis by Suppressing RAS-ERK and AKT/FOXO3a Pathways in Hepatocellular Carcinoma Cells. Chemistry & Biodiversity. 10.1002/cbdv.202300050
Zhang Huahua et al. (2022) Ribosomal protein RPL5 regulates colon cancer cell proliferation and migration through MAPK/ERK signaling pathway. BMC Molecular and Cell Biology. 10.1186/s12860-022-00448-z
