| Field | Specification |
|---|---|
| Target | |
| Alternative names | Forkhead box protein O4;Fork head domain transcription factor AFX1;FOXO4;AFX, AFX1, MLLT7; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human FoxO4 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HEI-6 is a rabbit monoclonal antibody that recognizes forkhead box protein O4 (gene FOXO4) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 53.7 kDa, and the supplier reports an observed band at about 65 kDa.
FOXO4 is a forkhead transcription factor of the FOXO group that is regulated by insulin–AKT signaling. It activates genes such as IGFBP1, dampens HIF-1α responses under low oxygen and contributes to cell cycle control and stress resistance. FOXO4 is studied in metabolism, cancer and cellular senescence.
| Target | Forkhead box protein O4 (gene FOXO4; UniProt P98177, human) |
|---|---|
| Clone | HEI-6 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 53.7 kDa |
| Observed MW | 65 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:5000–1:20000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human foxO4.
Reactivity Notes
The supplier lists reactivity with human. UniProt places forkhead box protein O4 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
