| Field | Specification |
|---|---|
| Target | |
| Alternative names | Forkhead box protein P3; Scurfin; Foxp3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of mouse Foxp3, which shares 94.4% amino acid (aa) sequence identity with human FOXP3. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets forkhead box protein P3 (gene Foxp3) in mouse and rat samples and is validated for Western blot. The predicted molecular weight is 47.3 kDa, and the supplier reports an observed band at about 50 kDa.
FOXP3 is a forkhead transcription factor that is the key lineage regulator of regulatory T cells (Tregs), which suppress immune responses and maintain tolerance. It is the most widely used Treg marker, and loss-of-function mutations cause IPEX syndrome in humans and the scurfy phenotype in mice. FOXP3 is studied in autoimmunity, transplantation and tumor immunology.
| Target | Forkhead box protein P3 (gene Foxp3; UniProt Q99JB6, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 47.3 kDa |
| Observed MW | 50 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|
Samples with a confirmed band (WB): rat thymus tissue, mouse spleen tissue, mouse thymus tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the middle region of mouse foxp3. Sequence identity with the human ortholog: 94.4%.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places forkhead box protein P3 in the nucleus and cytoplasm. Tissue expression noted by UniProt (mouse): High level of expression in thymus and spleen. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Yang Wang et al. (2020) Electroacupuncture Improves Blood Pressure in SHRs by Regulating the Immune Balance between Th17 and Treg. Evidence-based Complementary and Alternative Medicine. 10.1155/2020/5375981
Yang Xiao et al. (2014) Effect of Iodine Excess on Th1, Th2, Th17, and Treg Cell Subpopulations in the Thyroid of NOD.H-2h4 Mice. Biological Trace Element Research. 10.1007/s12011-014-9958-y
Dekui Xu et al. (2014) Salvia miltiorrhiza increases the expression of transcription factor Foxp3 in experimental murine colitis. Molecular Medicine Reports. 10.3892/mmr.2014.1986
