| Field | Specification |
|---|---|
| Target | |
| Alternative names | Forkhead box protein P3; Scurfin; FOXP3; IPEX |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human FOXP3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 17F26 is a rabbit monoclonal antibody that recognizes forkhead box protein P3 (gene FOXP3) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 47.2 kDa, and the supplier reports an observed band at about 47 kDa.
FOXP3 is a forkhead transcription factor that is the key lineage regulator of regulatory T cells (Tregs), which suppress immune responses and maintain tolerance. It is the most widely used Treg marker, and loss-of-function mutations cause IPEX syndrome in humans and the scurfy phenotype in mice. FOXP3 is studied in autoimmunity, transplantation and tumor immunology.
| Target | Forkhead box protein P3 (gene FOXP3; UniProt Q9BZS1, human) |
|---|---|
| Clone | 17F26 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 47.2 kDa |
| Observed MW | 47 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human squamous carcinoma tissue, human tongue cancer tissue, human Hodgkin's lymphoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human FOXP3.
Reactivity Notes
The supplier lists reactivity with human. UniProt places forkhead box protein P3 in the nucleus and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
