Anti-FOXRED2 Antibody Picoband®

Overview

Anti-FOXRED2 Antibody Picoband® is an antibody for FOXRED2 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: FOXRED2 (histone cluster 1 H3 family member a/b/c/d/e/f/g/h/i/j); UniProt: Q8IWF2
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 80 kDa
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-FOXRED2 Antibody Picoband® catalog # A12703-1.

Biological background

Biological context: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Expression and localization notes: cellular localization: Cytoplasm., tissue context: Expressed in fetal brain, fetal lung, fetal liver, heart, brain, placenta, lung, liver, muscle, kidney and pancreas..

Common research applications

• Western blotting (WB): Compare FOXRED2 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of FOXRED2 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify FOXRED2-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: FOXRED2, also known as ERFAD, is an endoplasmic reticulum (ER) luminal flavoprotein which may function in ER associated degradation (ERAD). It has been proposed to bind non-native proteins in the endoplasmic reticulum and target them to the ubiquitination machinery for subsequent degradation. Upon knockdown of FOXRED2, the degradation of the ERAD model substrate ribophorin 332 is delayed, and the overall level of polyubiquitinated cellular proteins is decreased. Defects in the related protein FOXRED1 are a cause of mitochondrial complex I deficiency (MT-C1D), suggesting the FOXRED2 may also play a role in MT-C1D.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cytoplasm.
• Tissue details: Expressed in fetal brain, fetal lung, fetal liver, heart, brain, placenta, lung, liver, muscle, kidney and pancreas.
• Research category: DNA/RNA,DNA Synthesis,Epigenetics and Nuclear Signaling,RNA Processing

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.