| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fos-related antigen 1; FRA-1; FOSL1; FRA1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human FRA1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24F71 is a rabbit monoclonal antibody that recognizes fos-related antigen 1 (gene FOSL1) in human samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 29.4 kDa, and the supplier reports an observed band at about 39 kDa.
Fra-1 (FOSL1) is a FOS family transcription factor that forms AP-1 complexes with JUN proteins. It is induced by growth factor and RAS–MAPK signaling and regulates genes involved in proliferation, migration and invasion. Fra-1 is often highly expressed in aggressive cancers and is studied in tumor progression and bone biology.
| Target | Fos-related antigen 1 (gene FOSL1; UniProt P15407, human) |
|---|---|
| Clone | 24F71 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 29.4 kDa |
| Observed MW | 39 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human MOLT-4 cells, human A431 cells, human U251 cells, human A549 cells, human SIHA cells, human U2OS cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human FRA1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places fos-related antigen 1 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Chuyu Wang et al. (2025) Juglone induces ferroptotic effect on hepatocellular carcinoma and pan-cancer via the FOSL1-HMOX1 axis. Phytomedicine. 10.1016/j.phymed.2025.156417
Zhiwei Yan et al. (2024) A novel signature constructed by mitochondrial function and cell death-related gene for the prediction of prognosis in bladder cancer. Scientific Reports. 10.1038/s41598-024-65594-0
Ji Qijian et al. (2025) Predictive and personalized approaches for idiopathic pulmonary fibrosis: a Wnt-related gene set scoring framework integrating single-cell sequencing, spatial transcriptomics, and machine learning for diagnosis and prognosis. Functional & Integrative Genomics. 10.1007/s10142-025-01571-8
Guangwei Zhong et al. (2015) Fra-1 is upregulated in lung cancer tissues and inhibits the apoptosis of lung cancer cells by the P53 signaling pathway. Oncology Reports. 10.3892/or.2015.4395
