| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fractalkine;C-X3-C motif chemokine 1;CX3C membrane-anchored chemokine;Neurotactin;Small-inducible cytokine D1;Processed fractalkine;Cx3cl1;Cx3c, Fkn, Scyd1; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of mouse CX3CL1, identical to the related rat sequence. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets fractalkine (gene Cx3cl1) in mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 42.1 kDa, and the supplier reports an observed band at about 90–100 kDa.
Fractalkine (CX3CL1) is the only member of the CX3C chemokine family. It exists as a membrane-anchored adhesion molecule on endothelial cells and neurons and as a soluble chemoattractant after cleavage. Acting through CX3CR1, it guides monocyte, NK cell and T-cell adhesion and migration, and it mediates neuron-microglia communication in the brain.
| Target | Fractalkine (gene Cx3cl1; UniProt O35188, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 42.1 kDa |
| Observed MW | 90–100 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse, Rat) |
|---|---|
| IHC (frozen sections) | 0.5–1 µg/mL (Mouse, Rat) |
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat) |
Samples with confirmed staining (IHC): rat intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide from the N-terminal region of mouse CX3CL1. The peptide sequence is conserved in rat.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places fractalkine in the cell membrane. Tissue expression noted by UniProt (mouse): Highest levels in brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
