| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interferon-induced transmembrane protein 3; Dispanin subfamily A member 2b; DSPA2b; Interferon-inducible protein 1-8U; IFITM3 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Fragilis |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADAC-9 is a rabbit monoclonal antibody that recognizes interferon-induced transmembrane protein 3 (gene IFITM3) in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 14.6 kDa, and the supplier reports an observed band at about 15 kDa.
IFITM3 (interferon-induced transmembrane protein 3, also called fragilis) sits in endosomal and lysosomal membranes and prevents enveloped viruses such as influenza A from fusing with them, thereby blocking release of the viral genome. It is one of the best-studied interferon effectors and a key protein in research on host restriction of viral entry.
| Target | Interferon-induced transmembrane protein 3 (gene IFITM3; UniProt Q01628, human) |
|---|---|
| Clone | ADAC-9 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 14.6 kDa |
| Observed MW | 15 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human fragilis.
Reactivity Notes
The supplier lists reactivity with human. UniProt places interferon-induced transmembrane protein 3 in the cell membrane, late endosome membrane, early endosome membrane and lysosome membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
