| Field | Specification |
|---|---|
| Target | |
| Alternative names | Proto-oncogene FRAT1; Frequently rearranged in advanced T-cell lymphomas 1; FRAT-1; FRAT1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human FRAT1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 28F68 is a rabbit monoclonal antibody that recognizes proto-oncogene FRAT1 in human, mouse and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 29.1 kDa, and the supplier reports an observed band at about 29 kDa.
FRAT1 is a GSK-3-binding protein that promotes Wnt signaling. By binding GSK-3 it limits phosphorylation of β-catenin, allowing β-catenin to accumulate and activate Wnt target genes. It was first identified as a proto-oncogene in mouse T-cell lymphomas and is studied in Wnt pathway regulation and cancer biology.
| Target | Proto-oncogene FRAT1 (gene FRAT1; UniProt Q92837, human) |
|---|---|
| Clone | 28F68 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 29.1 kDa |
| Observed MW | 29 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human FRAT1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places proto-oncogene FRAT1 in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
