| Field | Specification |
|---|---|
| Target | |
| Alternative names | Frizzled-8; Fz-8; hFz8; FZD8 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Frizzled 8 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20F28 is a rabbit monoclonal antibody that recognizes frizzled-8 (gene FZD8) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 73.3 kDa, and the supplier reports an observed band at about 73 kDa.
Frizzled-8 (FZD8) is a seven-pass transmembrane Wnt receptor that, with LRP5 or LRP6, activates canonical Wnt/β-catenin signaling. It is studied in embryonic development and in several cancers, and its cysteine-rich domain has served as a model for how Wnt ligands bind frizzled receptors.
| Target | Frizzled-8 (gene FZD8; UniProt Q9H461, human) |
|---|---|
| Clone | 20F28 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 73.3 kDa |
| Observed MW | 73 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Immunogen
Synthetic peptide based on the sequence of human frizzled 8.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places frizzled-8 in the membrane, Golgi apparatus and cell membrane. Tissue expression noted by UniProt (human): Most abundant in fetal kidney, followed by brain and lung.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ji Qijian et al. (2025) Predictive and personalized approaches for idiopathic pulmonary fibrosis: a Wnt-related gene set scoring framework integrating single-cell sequencing, spatial transcriptomics, and machine learning for diagnosis and prognosis. Functional & Integrative Genomics. 10.1007/s10142-025-01571-8
