| Field | Specification |
|---|---|
| Target | |
| Alternative names | FZD9; FZD3; Frizzled-9; Fz-9; hFz9; FzE6; CD antigen CD349 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Frizzled 9/FZD9 recombinant protein (Position: R27-L591). Human Frizzled 9/FZD9 shares 95.9% and 96.1% amino acid (aa) sequence identity with mouse and rat Frizzled 9/FZD9, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets frizzled-9 (gene FZD9) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA.
Frizzled-9 (FZD9) is a Wnt receptor of the frizzled family expressed in brain, testis and other tissues. It has been linked to neural development, hippocampal function, bone formation and B-cell development. FZD9 lies in the region deleted in Williams syndrome.
| Target | Frizzled-9 (gene FZD9; UniProt O00144, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human Frizzled 9/FZD9 fragment (Arg27–Leu591), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 95.9% and 96.1%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places frizzled-9 in the cell membrane. Tissue expression noted by UniProt (human): Expressed predominantly in adult and fetal brain, testis, eye, skeletal muscle and kidney.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
