| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fibroblast growth factor receptor substrate 2; FGFR substrate 2; FGFR-signaling adaptor SNT; Suc1-associated neurotrophic factor target 1; SNT-1; FRS2 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human FRS2 Adapter protein that links FGR and NGF receptors to downstream signaling pathways. Involved in the activation of MAP kinases. Modulates signaling via SHC1 by competing for a common binding site on NTRK1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACOG-6 is a rabbit monoclonal antibody that recognizes fibroblast growth factor receptor substrate 2 (gene FRS2) in human samples and is validated for Western blot, immunoprecipitation and flow cytometry. The predicted molecular weight is 57.0 kDa, and the supplier reports an observed band at about 75 kDa.
FRS2 (FRS2α) is a membrane-anchored docking protein that is phosphorylated by activated FGF receptors and by the NGF receptor TrkA. Once phosphorylated, it recruits GRB2 and SHP2 to switch on Ras–MAPK and PI3K–AKT signaling, making it a central adaptor in studies of FGF signaling, development and FGFR-driven cancers.
| Target | Fibroblast growth factor receptor substrate 2 (gene FRS2; UniProt Q8WU20, human) |
|---|---|
| Clone | ACOG-6 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 57.0 kDa |
| Observed MW | 75 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human FRS2.
Reactivity Notes
The supplier lists reactivity with human. UniProt places fibroblast growth factor receptor substrate 2 in the endomembrane system. Tissue expression noted by UniProt (human): Highly expressed in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
