| Field | Specification |
|---|---|
| Target | |
| Alternative names | ATP-dependent 6-phosphofructokinase, muscle type; ATP-PFK; PFK-M; 6-phosphofructokinase type A; Phosphofructo-1-kinase isozyme A; PFK-A; Phosphohexokinase; PFKM; PFKX |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Fructose 6 Phosphate Kinase |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18P49 is a rabbit monoclonal antibody that recognizes ATP-dependent 6-phosphofructokinase, muscle type (gene PFKM) in human, mouse and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 85.2 kDa, and the supplier reports an observed band at about 81 kDa.
PFKM is the muscle isoform of phosphofructokinase-1, which catalyzes the ATP-dependent phosphorylation of fructose 6-phosphate, the first committed step of glycolysis. It is the only PFK-1 isoform in skeletal muscle, and its inherited deficiency causes glycogen storage disease type VII (Tarui disease) with exercise intolerance.
| Target | ATP-dependent 6-phosphofructokinase, muscle type (gene PFKM; UniProt P08237, human) |
|---|---|
| Clone | 18P49 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 85.2 kDa |
| Observed MW | 81 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:100 |
Samples with a confirmed band (WB): human HeLa cells, human MCF-7 cells, human SH-SY5Y cells, rat heart tissue, rat brain tissue, mouse heart tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human fructose 6 Phosphate Kinase.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ATP-dependent 6-phosphofructokinase, muscle type in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhihui Song et al. (2024) Integrated metabolomic and transcriptomic analysis reveals the effects and mechanisms of Jinqi Jiangtang tablets on type 2 diabetes. Phytomedicine. 10.1016/j.phymed.2024.155957
