| Field | Specification |
|---|---|
| Target | |
| Alternative names | ATP-dependent RNA helicase me31b; Maternal expression at 31B; me31B |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Conjugate | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets ATP-dependent RNA helicase me31b in Fruit fly samples and is validated for Western blot.
In Drosophila, Me31B is a DEAD-box RNA helicase and a core component of ribonucleoprotein granules such as P-bodies. Working with partners such as Tral and Cup, it represses translation of maternal mRNAs during oogenesis and early embryogenesis and promotes mRNA decapping. It is a model protein for studying translational control, RNA granules and germline development.
| Target | ATP-dependent RNA helicase me31b (gene me31B; UniProt P23128, Drosophila) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Fruit fly |
| Form | Liquid |
| Formulation | 20 mM PBS; 50% glycerol; 0.02% NaN3 |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:10,000 |
|---|
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, primary antibody at 1:10 overnight at 4 °C, HRP-conjugated secondary antibody at 1:10, ECL detection.
Reactivity Notes
The supplier lists reactivity with Fruit fly. UniProt places ATP-dependent RNA helicase me31b in the cytoplasm, endoplasmic reticulum and cell projection. Tissue expression noted by UniProt (Drosophila): Ubiquitously expressed throughout the brain (at protein level).
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
BrineyChloe A et al. (2025) Muskelin is a substrate adaptor of the highly regulated Drosophila embryonic CTLH E3 ligase. EMBO Reports. 10.1038/s44319-025-00397-6
