| Field | Specification |
|---|---|
| Target | |
| Alternative names | Follicle-stimulating hormone receptor;FSH-R;Follitropin receptor;FSHR;LGR1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human FSH Receptor recombinant protein (Position: C18-N187). Human FSH Receptor shares 91.2% and 90% amino acid (aa) sequence identity with mouse and rat FSH Receptor, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets follicle-stimulating hormone receptor (gene FSHR) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 78.3 kDa, and the supplier reports an observed band at about 78 kDa.
The follicle-stimulating hormone receptor (FSHR) is a G protein-coupled receptor on granulosa cells in the ovary and Sertoli cells in the testis. Binding of FSH signals mainly through cAMP and protein kinase A, together with PI3K–AKT, to drive follicle development and steroid hormone production. Reported actions outside the gonads, for example on bone cells, are also under study.
| Target | Follicle-stimulating hormone receptor (gene FSHR; UniProt P23945, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 78.3 kDa |
| Observed MW | 78 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): rat testis tissue, rat ovary tissue, mouse testis tissue, mouse ovary tissue, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human FSH Receptor fragment (Cys18–Asn187), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 91.2% and 90%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places follicle-stimulating hormone receptor in the cell membrane. Tissue expression noted by UniProt (human): Sertoli cells and ovarian granulosa cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Weidong Zhou et al. (2024) Nano-sized titanium dioxide exposure induces non-hyperandrogenic polycystic ovarian syndrome in rats by increasing aromatase and apoptosis in granulosa cells. Journal of Environmental Sciences. 10.1016/j.jes.2024.10.009
Weihan Yang et al. (2022) Quercetin Alleviates Endoplasmic Reticulum Stress-Induced Apoptosis in Buffalo Ovarian Granulosa Cells. Animals. 10.3390/ani12060787
Dingqing Feng et al. (2017) Gonadotropins promote human ovarian cancer cell migration and invasion via a cyclooxygenase 2-dependent pathway. Oncology Reports. 10.3892/or.2017.5784
Gong Zhuandi et al. (2018) FSH receptor binding inhibitor restrains follicular development and possibly attenuates carcinogenesis of ovarian cancer through down-regulating expression levels of FSHR and ERβ in normal ovarian tissues. Gene. 10.1016/j.gene.2018.05.068
Liu Shengnan et al. (2020) Effect of miR-26b-5p on cis-diamine dichloroplatinum–induced ovarian granulosa cell injury by targeting MAP3K9. In Vitro Cellular & Developmental Biology-Animal. 10.1007/s11626-020-00439-4
