| Field | Specification |
|---|---|
| Target | |
| Alternative names | Follicle-stimulating hormone receptor;FSH-R;Follitropin receptor;FSHR;LGR1; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human FSH Receptor, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets follicle-stimulating hormone receptor (gene FSHR) in human and mouse samples and is validated for Western blot. The predicted molecular weight is 78.3 kDa, and the supplier reports an observed band at about 78 kDa.
The follicle-stimulating hormone receptor (FSHR) is a G protein-coupled receptor on granulosa cells in the ovary and Sertoli cells in the testis. Binding of FSH signals mainly through cAMP and protein kinase A, together with PI3K–AKT, to drive follicle development and steroid hormone production. Reported actions outside the gonads, for example on bone cells, are also under study.
| Target | Follicle-stimulating hormone receptor (gene FSHR; UniProt P23945, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 78.3 kDa |
| Observed MW | 78 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse) |
|---|
Samples with a confirmed band (WB): mouse ovary tissue, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Synthetic peptide from the middle region of human FSH Receptor. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places follicle-stimulating hormone receptor in the cell membrane. Tissue expression noted by UniProt (human): Sertoli cells and ovarian granulosa cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Cong Liu et al. (2013) Female hormone release of microencapsulated Xenopus laevis ovarian cells. International Journal of Pharmaceutics. 10.1016/j.ijpharm.2013.04.048
Luo Qianqian et al. (2020) The Effects of Inhibin B in the Chemotherapy Drug-Induced Premature Ovarian Insufficiency Mice and hPMSCs Treatment. Reproductive Sciences. 10.1007/s43032-019-00128-y
Jing Wu et al. (2013) T-2 toxin exposure induces apoptosis in rat ovarian granulosa cells through oxidative stress. Environmental Toxicology and Pharmacology. 10.1016/j.etap.2013.03.017
Jia Liu et al. (2014) Serum levels of sex hormones and expression of their receptors in thyroid tissue in female patients with various types of thyroid neoplasms. Pathology Research and Practice. 10.1016/j.prp.2014.09.002
