| Field | Specification |
|---|---|
| Target | |
| Alternative names | RNA-binding protein FUS;75 kDa DNA-pairing protein;Oncogene FUS;Oncogene TLS;POMp75;Translocated in liposarcoma protein;FUS;TLS; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human FUS / TLS |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IHC-6 is a rabbit monoclonal antibody that recognizes RNA-binding protein FUS in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 53.4 kDa, and the supplier reports an observed band at about 70 kDa.
FUS is a nuclear DNA/RNA-binding protein of the FET family that takes part in transcription, splicing, mRNA transport and the DNA damage response. It has a low-complexity domain that can drive liquid–liquid phase separation. Mutations in FUS cause some forms of amyotrophic lateral sclerosis (ALS), and FUS inclusions are seen in a subset of frontotemporal lobar degeneration.
| Target | RNA-binding protein FUS (gene FUS; UniProt P35637, human) |
|---|---|
| Clone | IHC-6 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 53.4 kDa |
| Observed MW | 70 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human K562 cells, human HepG2 cells, human HeLa cells, human Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human FUS / TLS.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places RNA-binding protein FUS in the nucleus. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yuzhen Sui et al. (2024) BHBA attenuates endoplasmic reticulum stress-dependent neuroinflammation via the gut–brain axis in a mouse model of heat stress. CNS Neuroscience & Therapeutics. 10.1111/cns.14840
