| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Ras-related protein Rab-11B; GTP-binding protein YPT3; RAB11B; YPT3 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human FUT4, which shares 72.7% and 71.4% amino acid (aa) sequence identity with mouse and rat FUT4, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-FUT4 Antibody Picoband® is an antibody for FUT4 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: FUT4 (RAB11B, member RAS oncogene family); UniProt: P22083
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 70 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-FUT4 Antibody catalog # A04535-1.
Biological background
Biological context: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors ly responsible for vesicle formation, movement, tethering and fusion. That Rab plays a role in endocytic recycling, regulating apical recycling of several transmembrane proteins including cystic fibrosis transmembrane conductance regulator/CFTR, epithelial sodium channel/ENaC, potassium voltage-gated channel, and voltage-dependent L-type calcium channel. May also regulate constitutive and regulated secretion, like insulin granule exocytosis. Required for melanosome transport and release from melanocytes. Also regulates V-ATPase intracellular transport in response to extracellular acidosis.
Expression and localization notes: cellular localization: Recycling endosome membrane, Lipid-anchor, Synaptic vesicle membrane, Phagosome membrane., tissue context: Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas..
Common research applications
- Western blotting (WB): Compare FUT4 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of FUT4 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify FUT4-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific), also known as FUT4, is an enzyme which in humans is encoded by the FUT4 gene. The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15).
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Recycling endosome membrane, Lipid-anchor, Synaptic vesicle membrane, Phagosome membrane.
- Tissue details: Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.
- Research category: Angiogenesis,Cancer,Cardiovascular,Growth Factors,Growth Factors/Hormones,Invasion/Microenvironment,Neurogenesis,Neurology Process,Neuroscience,Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
