| Field | Specification |
|---|---|
| Target | |
| Alternative names | Alpha-; 1,3; -fucosyltransferase 4; 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase; ELAM-1 ligand fucosyltransferase; Fucosyltransferase 4; Fucosyltransferase IV; Fuc-TIV; FucT-IV; Galactoside 3-L-fucosyltransferase; FUT4; ELFT; FCT3A |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human FUT4, which shares 72.7% and 71.4% amino acid (aa) sequence identity with mouse and rat FUT4, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets alpha-(1,3)-fucosyltransferase 4 (gene FUT4) in human samples and is validated for Western blot. The predicted molecular weight is 59.1 kDa, and the supplier reports an observed band at about 70 kDa.
FUT4 is an α(1,3)-fucosyltransferase that fucosylates N-acetylglucosamine in lactosamine chains, producing the Lewis X antigen (also known as SSEA-1 or CD15). This glycan epitope marks myeloid cells and some stem cell populations, and FUT4 is studied in leukocyte biology, development and cancer.
| Target | Alpha-(1,3)-fucosyltransferase 4 (gene FUT4; UniProt P22083, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 59.1 kDa |
| Observed MW | 70 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human HL-60 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human FUT4. Sequence identity with the mouse and rat orthologs: 72.7% and 71.4%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places alpha-(1,3)-fucosyltransferase 4 in the Golgi apparatus. Tissue expression noted by UniProt (human): Expressed at low levels in bone marrow-derived mesenchymal stem cells... The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
