| Field | Specification |
|---|---|
| Target | |
| Alternative names | RNA-binding protein FXR1; FMR1 autosomal homolog 1; hFXR1p; FXR1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human FXR1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25F02 is a rabbit monoclonal antibody that recognizes RNA-binding protein FXR1 in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 69.7 kDa, and the supplier reports an observed band at about 70–80 kDa.
FXR1 is an RNA-binding protein of the fragile X protein family, related to FMRP and FXR2. It binds target mRNAs, including AU-rich elements in 3′-UTRs, to regulate their translation and stability. FXR1 is highly expressed in muscle and testis and is studied in muscle development, spermatogenesis, neural function and cancer.
| Target | RNA-binding protein FXR1 (gene FXR1; UniProt P51114, human) |
|---|---|
| Clone | 25F02 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 69.7 kDa |
| Observed MW | 70–80 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human FXR1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places RNA-binding protein FXR1 in the cytoplasm, cell projection, nucleus envelope and postsynapse. Tissue expression noted by UniProt (human): Expressed in all tissues examined including heart, brain, kidney and testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Weichun Xie et al. (2026) Gut Microbiota-Derived Ursodeoxycholic Acid Mediates the Resistance to Colonic Inflammation in Pigs. Journal of Agricultural and Food Chemistry. 10.1021/acs.jafc.5c08687
Guinan Xie et al. (2026) Jiangzhi ruanmai recipe alleviates atherosclerosis in ApoE−/− mice by regulating cholesterol metabolism involved in gut microbiota remodeling. Frontiers in Cardiovascular Medicine. 10.3389/fcvm.2026.1834983
