| Field | Specification |
|---|---|
| Target | |
| Alternative names | RNA-binding protein FXR2; FXR2P; FMR1 autosomal homolog 2; FXR2; FMR1L2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human FXR2 recombinant protein (Position: N153-K651). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects RNA-binding protein FXR2 in human, monkey and mouse samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 74.2 kDa, and the supplier reports an observed band at about 93 kDa.
FXR2 is an RNA-binding protein of the fragile X protein family, closely related to FMRP and FXR1. It associates with polysomes and controls translation and stability of target mRNAs, and in mouse models it is needed for normal adult hippocampal neurogenesis. FXR2 is studied in neural development and fragile X-related biology.
| Target | RNA-binding protein FXR2 (gene FXR2; UniProt P51116, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Monkey, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 74.2 kDa |
| Observed MW | 93 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Monkey) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human, Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): monkey COS-7 cells, huamn U251 cells, human SH-SY5Y cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): HEPA1–6 cells, RT4 cells.
Immunogen
Recombinant human FXR2 fragment (Asn153–Lys651), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, monkey and mouse. UniProt places RNA-binding protein FXR2 in the cytoplasm and postsynapse. Tissue expression noted by UniProt (human): Expressed in all tissues examined including heart, brain, kidney and testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
