| Field | Specification |
|---|---|
| Target | |
| Alternative names | FYN-binding protein 1; Adhesion and degranulation promoting adaptor protein; ADAP; FYB-120/130; p120/p130; FYN-T-binding protein; SLAP-130; SLP-76-associated phosphoprotein; FYB1; FYB; SLAP130 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human FYB |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20F94 is a rabbit monoclonal antibody that recognizes FYN-binding protein 1 (gene FYB1) in human and mouse samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 85.4 kDa, and the supplier reports an observed band at about 120 kDa.
FYB1 (also called ADAP or SLAP-130) is an adaptor protein in T cells and platelets that links antigen receptor signaling to integrin activation. Working with SLP-76 (LCP2) and SKAP1, it supports T-cell adhesion and cytoskeletal remodeling, and it also contributes to platelet function. It is studied in immune cell signaling.
| Target | FYN-binding protein 1 (gene FYB1; UniProt O15117, human) |
|---|---|
| Clone | 20F94 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 85.4 kDa |
| Observed MW | 120 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human TF-1 cells, human Jurkat cells, human HEL cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human FYB.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places FYN-binding protein 1 in the cytoplasm, nucleus and cell junction. Tissue expression noted by UniProt (human): Expressed in hematopoietic tissues such as myeloid and T-cells, spleen and thymus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
