| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Glucose-6-phosphate 1-dehydrogenase;G6PD;1.1.1.49;G6PD; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human G6PD |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal Antibody is an antibody targeting G6PD. Common applications include WB, IHC, ICC, IF, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: ABBI-7; isotype: Rabbit IgG; reactivity: Human; observed MW: 59 kDa; calculated MW: 59257 MW.
Boster Bio Anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal Antibody catalog # M00287. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: G6PD — Glucose-6-phosphate 1-dehydrogenase
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: ABBI-7; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 59 kDa; Calculated: 59257 MW
Biological background
Protein function (datasheet): Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis. .
Cellular localization (datasheet): Isoform 1: Secreted, extracellular space, extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
Tissue details (datasheet): Isoform Long is found in lymphoblasts, granulocytes and sperm.
Research relevance and current trends
- Commonly studied in contexts related to Atherosclerosis,Cancer,Cancer Metabolism,Carbohydrate Metabolism,Cardiovascular,Diabetes,Diabetes-associated,Energy Metabolism,Energy Transfer Pathways,Heart Disease,Metabolic Signaling Pathway,Metabolic Signaling Pathways,Metabolism,Metabolism Of Carbohydrates,Pathways and Processes,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
