| Field | Specification |
|---|---|
| Target | |
| Alternative names | Lysosomal alpha-glucosidase; Acid maltase; Aglucosidase alfa; GAA |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GAA |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25G46 is a rabbit monoclonal antibody that recognizes lysosomal alpha-glucosidase (gene GAA) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 105.3 kDa; the supplier reports observed bands at 110 kDa, 95 kDa, 76 kDa.
Lysosomal acid α-glucosidase (GAA, acid maltase) breaks down glycogen inside lysosomes by cleaving α-1,4 and, more slowly, α-1,6 glucose linkages. Loss of GAA activity causes Pompe disease (glycogen storage disease type II), in which glycogen builds up in muscle, so the enzyme is a focus of lysosomal and neuromuscular research.
| Target | Lysosomal alpha-glucosidase (gene GAA; UniProt P10253, human) |
|---|---|
| Clone | 25G46 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 105.3 kDa |
| Observed MW | 110 kDa, 95 kDa, 76 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Immunogen
Synthetic peptide based on the sequence of human GAA.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places lysosomal alpha-glucosidase in the lysosome and lysosome membrane.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
