| Field | Specification |
|---|---|
| Target | |
| Alternative names | GRB2-associated-binding protein 2; GRB2-associated binder 2; Growth factor receptor bound protein 2-associated protein 2; pp100; GAB2; KIAA0571 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human GAB2 recombinant protein (Position: S162-R504). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes GRB2-associated-binding protein 2 (gene GAB2) in human and mouse samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 74.5 kDa, and the supplier reports an observed band at about 90 kDa.
GAB2 is a docking protein that assembles signaling complexes downstream of cytokine, antigen, growth factor and other receptors. By recruiting SHP2 and PI3K it boosts MAPK and PI3K–AKT signaling, and it is needed for mast cell activation in allergic responses and for osteoclast development. GAB2 is also studied in leukemia and breast cancer.
| Target | GRB2-associated-binding protein 2 (gene GAB2; UniProt Q9UQC2, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 74.5 kDa |
| Observed MW | 90 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human K562 cells, human SH-SY5Y cells, mouse ovary tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): HL-60 cells, THP-1 cells.
Immunogen
Recombinant human GAB2 fragment (Ser162–Arg504), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places GRB2-associated-binding protein 2 in the cytoplasm, cell membrane and membrane raft. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
