| Field | Specification |
|---|---|
| Target | |
| Alternative names | Gamma-aminobutyric acid type B receptor subunit 2; GABA-B receptor 2; GABA-B-R2; GABA-BR2; GABABR2; Gb2; G protein-coupled receptor 51; HG20; GABBR2; GPR51; GPRC3B |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GABA B Receptor 2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18G32 is a rabbit monoclonal antibody that recognizes gamma-aminobutyric acid type B receptor subunit 2 (gene GABBR2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 105.8 kDa, and the supplier reports an observed band at about 106 kDa.
GABBR2 is one of the two subunits of the GABA(B) receptor, the G protein-coupled receptor for GABA. Within the GABBR1–GABBR2 heterodimer, GABBR2 does not bind GABA but carries the receptor to the cell surface and couples it to Gi/o proteins. GABA(B) receptors produce slow synaptic inhibition and are studied in neuroscience and pharmacology.
| Target | Gamma-aminobutyric acid type B receptor subunit 2 (gene GABBR2; UniProt O75899, human) |
|---|---|
| Clone | 18G32 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 105.8 kDa |
| Observed MW | 106 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:40 |
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human GABA B Receptor 2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places gamma-aminobutyric acid type B receptor subunit 2 in the cell membrane and postsynaptic cell membrane. Tissue expression noted by UniProt (human): Highly expressed in brain, especially in cerebral cortex, thalamus, hippocampus, frontal, occipital and temporal lobe, occipital pole and cerebellum, followed by corpus callosum, caudate nucleus, spinal cord, amygdala and medulla. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
