| Field | Specification |
|---|---|
| Target | |
| Alternative names | Gamma-aminobutyric acid receptor-associated protein; GABA(A) receptor-associated protein; MM46; GABARAP; FLC3B |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GABARAP GABARAP is cleaved at its carboxyl terminus, which leads to conjugation by either of the phospholipids phosphatidylethanolamine or phosphatidylserine. This processing converts GABARAP from a type I to a type II membrane bound form involved in autophagosome biogenesis. Processing of GABARAP involves cleavage by Atg4 family members followed by conjugation by the E1 and E2 like enzymes Atg7 and Atg3. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACHE-7 is a rabbit monoclonal antibody that recognizes gamma-aminobutyric acid receptor-associated protein (gene GABARAP) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 13.9 kDa, and the supplier reports an observed band at about 18 kDa.
GABARAP is a ubiquitin-like protein of the ATG8 family. It was first found to bind GABA(A) receptors and help their trafficking, and it is also conjugated to phosphatidylethanolamine on autophagic membranes, where GABARAP-subfamily proteins are important for late steps of autophagosome formation. GABARAP is studied in autophagy, mitophagy and neuronal receptor transport.
| Target | Gamma-aminobutyric acid receptor-associated protein (gene GABARAP; UniProt O95166, human) |
|---|---|
| Clone | ACHE-7 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 13.9 kDa |
| Observed MW | 18 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:10000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human U87 cells, human U251 cells, human HepG2 cells, human HUH7 cells, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic phosphopeptide based on human GABARAP GABARAP is cleaved at its carboxyl terminus, which leads to conjugation by either of the phospholipids phosphatidylethanolamine or phosphatidylserine.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places gamma-aminobutyric acid receptor-associated protein in the endomembrane system, cytoplasm, Golgi apparatus membrane and cytoplasmic vesicle. Tissue expression noted by UniProt (human): Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Qu Yinmeng et al. (2025) FCGR2B knockdown alleviates diabetes-induced cognitive dysfunction by altering neuronal excitability. Molecular Medicine. 10.1186/s10020-025-01301-7
