| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Galectin-1; Gal-1; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; Galaptin; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; S-Lac lectin 1; Lgals1; Gbp; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived mouse Galectin 1 recombinant protein (Position: A2-E135). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of Lgals1 (Tumor necrosis factor ligand superfamily member 10) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Galectin 1/Lgals1 Antibody Picoband® catalog # A00470. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived mouse Galectin 1 recombinant protein (Position: A2-E135). (reported region: A2-E135).
- Molecular weight context: reported MW: 15 kDa; calculated MW: 33477 MW
- Reactivity: Mouse,Rat
- Applications: ELISA, IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Tumor necrosis factor ligand superfamily member 10; lectin, galactose binding, soluble 1. Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Galectin-1 expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ T-cells. It is thought to play a role in creating immune tolerance in pregnancy. It has been found that Galectin-1-mediated production of IL6 may assist in augmenting the innate immune response against NiV. Galectin-1 may also be a significant factor that augments the efficiency of the HIV-1 infection process. Functional note: Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis. Reported localization: Secreted, extracellular space, extracellular matrix Expression/tissue context: Widespread; most predominant in spleen, lung and prostate.
Research relevance and current trends
- Mechanistic pathway studies: Researchers commonly examine how Lgals1 (Tumor necrosis factor ligand superfamily member 10) relates to this theme using model systems and orthogonal readouts.
- Biomarker profiling across models: Researchers commonly examine how Lgals1 (Tumor necrosis factor ligand superfamily member 10) relates to this theme using model systems and orthogonal readouts.
- Perturbation-response experiments (time-course/dose–response): Researchers commonly examine how Lgals1 (Tumor necrosis factor ligand superfamily member 10) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative Lgals1 (Tumor necrosis factor ligand superfamily member 10) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of Lgals1 (Tumor necrosis factor ligand superfamily member 10) across tissue regions and cell types using matched controls.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
