| Field | Specification |
|---|---|
| Target | |
| Alternative names | Galectin-1; Gal-1; 14 kDa laminin-binding protein; HLBP14; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; Galaptin; HBL; HPL; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; Putative MAPK-activating protein PM12; S-Lac lectin 1; LGALS1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Galectin 1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 28L34 is a rabbit monoclonal antibody that recognizes galectin-1 (gene LGALS1) in human, mouse and rat samples and is validated for Western blot, IHC, flow cytometry and immunoprecipitation. The predicted molecular weight is 14.7 kDa, and the supplier reports an observed band at about 14 kDa.
Galectin-1 (LGALS1) is a small β-galactoside-binding lectin that is secreted and found both inside and outside cells. By cross-linking glycoproteins on the cell surface, it regulates cell adhesion, proliferation and apoptosis, and it strongly dampens T-cell responses, including by inducing apoptosis of activated T cells. Galectin-1 is studied in immune regulation, angiogenesis and tumor immune evasion.
| Target | Galectin-1 (gene LGALS1; UniProt P09382, human) |
|---|---|
| Clone | 28L34 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 14.7 kDa |
| Observed MW | 14 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): 293 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human galectin 1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes galectin-1 as secreted and also located in the cytoplasm. Tissue expression noted by UniProt (human): Expressed in placenta, maternal decidua and fetal membranes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ye Zhanhong et al. (2020) Galectins, Eosinophiles, and Macrophages May Contribute to Schistosoma japonicum Egg-Induced Immunopathology in a Mouse Model. Frontiers in Immunology. 10.3389/fimmu.2020.00146
