| Field | Specification |
|---|---|
| Target | |
| Alternative names | Gamma-synuclein; Breast cancer-specific gene 1 protein; Persyn; Synoretin; SR; SNCG; BCSG1; PRSN |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human gamma Synuclein/SNCG, which shares 53.8% amino acid (aa) sequence identity with rat SNCG. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects gamma-synuclein (gene SNCG) in human samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 13.3 kDa, and the supplier reports an observed band at about 17 kDa.
Gamma-synuclein (SNCG) is a synuclein family protein expressed mainly in peripheral neurons. It is thought to help maintain the neurofilament network and axonal architecture, and it is also studied for its overexpression in several cancers.
| Target | Gamma-synuclein (gene SNCG; UniProt O76070, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 13.3 kDa |
| Observed MW | 17 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HeLa cells, human RT4 cells, human Hacat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): HeLa cells.
Immunogen
Synthetic peptide from the C-terminal region of human gamma Synuclein/SNCG. Sequence identity with the rat ortholog: 53.8%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places gamma-synuclein in the cytoplasm. Tissue expression noted by UniProt (human): Highly expressed in brain, particularly in the substantia nigra. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
