| Field | Specification |
|---|---|
| Target | |
| Alternative names | Neuromodulin; Axonal membrane protein GAP-43; Growth-associated protein 43; Neural phosphoprotein B-50; pp46; GAP43 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse GAP43 recombinant protein (Position: M1-A227). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects neuromodulin (gene Gap43) in human, mouse and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 23.6 kDa, and the supplier reports an observed band at about 45 kDa.
GAP-43 (neuromodulin) is a neuron-specific membrane protein that is abundant in axonal growth cones during development and regeneration. It binds calmodulin and is a substrate of protein kinase C, and it regulates actin dynamics during axon growth and synaptic plasticity. GAP-43 is widely used as a marker of axon growth and regeneration in mammals.
| Target | Neuromodulin (gene Gap43; UniProt P06837, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 23.6 kDa |
| Observed MW | 45 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue, mouse C6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): Raji cells.
Immunogen
Recombinant mouse GAP43 fragment (Met1–Ala227), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places neuromodulin in the cell membrane, cell projection and synapse. Tissue expression noted by UniProt (mouse): Expressed in the hippocampus (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Dingyu Wang et al. (2015) Regulation of Intracellular Structural Tension by Talin in the Axon Growth and Regeneration. Molecular Neurobiology. 10.1007/s12035-015-9394-9
Zhong Juan et al. (2024) Low-Dose LPS Modulates Microglia/Macrophages Phenotypic Transformation to Amplify Rehabilitation Effects in Chronic Spinal Cord Injured (CSCI) Mice. Molecular Neurobiology. 10.1007/s12035-024-03979-y
Liu Hui et al. (2017) Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo. Scientific Reports. 10.1038/srep39869
Wang Yunhang et al. (2023) Osteopontin enhances the effect of treadmill training and promotes functional recovery after spinal cord injury. Molecular Biomedicine. 10.1186/s43556-023-00154-y
Yunbo Chen et al. (2014) β-Asarone prevents autophagy and synaptic loss by reducing ROCK expression in asenescence-accelerated prone 8 mice. Brain Research. 10.1016/j.brainres.2014.01.005
Zhengjiang Liu et al. (2018) Chronic Unpredictable Mild Stress Causing Cardiac and Thoracic Spinal Cord Electrophysiological Abnormalities May Be Associated with Increased Cardiac Expression of Serotonin and Growth-Associated Protein-43 in Rats. Biomed Research International. 10.1155/2018/8697913
Tao Tao et al. (2016) Minocycline Promotes Neurite Outgrowth of PC12 Cells Exposed to Oxygen-Glucose Deprivation and Reoxygenation Through Regulation of MLCP/MLC Signaling Pathways. Cellular and Molecular Neurobiology. 10.1007/s10571-016-0374-z
Jing Liu et al. (2006) Catalpol increases hippocampal neuroplasticity and up-regulates PKC and BDNF in the aged rats. Brain Research. 10.1016/j.brainres.2006.09.058
Qing-Fei Niu et al. (2018) Adsorptive properties of graphene oxide on vitamin B12 and their effect on the promotion of peripheral nerve regeneration. Neurological Research. 10.1080/01616412.2018.1557868
Quan Yuan et al. (2017) Human microvascular endothelial cell promotes the development of dorsal root ganglion neurons via BDNF pathway in a co-culture system. Bioscience Biotechnology and Biochemistry. 10.1080/09168451.2017.1313695
