| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glyceraldehyde-3-phosphate dehydrogenase;GAPDH;1.2.1.12;Peptidyl-cysteine S-nitrosylase GAPDH;2.6.99.-;GAPDH;GAPD;CDABP0047, OK/SW-cl.12; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GAPDH |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BH-7 is a rabbit monoclonal antibody that recognizes glyceraldehyde-3-phosphate dehydrogenase (gene GAPDH) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 36.1 kDa, and the supplier reports an observed band at about 36 kDa.
GAPDH catalyzes a central step of glycolysis, the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. Because it is abundant and broadly expressed, it is one of the most widely used loading controls for Western blot, although it also has non-glycolytic roles in the nucleus, in membrane trafficking and in translational control.
| Target | Glyceraldehyde-3-phosphate dehydrogenase (gene GAPDH; UniProt P04406, human) |
|---|---|
| Clone | BH-7 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 36.1 kDa |
| Observed MW | 36 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:5000–1:50000 |
|---|---|
| Immunohistochemistry | 1:100–1:500 |
| Immunocytochemistry / Immunofluorescence | 1:100–1:250 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with confirmed staining (IHC): rat colon, mouse testis, human uterus, human kidney tissue, human pituitary adenoma tissue, human squamous carcinoma tissue, human testis cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human GAPDH.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places glyceraldehyde-3-phosphate dehydrogenase in the cytoplasm, nucleus and membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Su Xi et al. (2024) Autophagy defects at weaning impair complement-dependent synaptic pruning and induce behavior deficits. Journal of Neuroinflammation. 10.1186/s12974-024-03235-z
Osman Sajeda et al. (2026) Quercetin-loaded exosomes derived from human umbilical cord mesenchymal stem cells alleviate microglia-mediated neuroinflammation via an anti-inflammatory mechanism. Inflammopharmacology. 10.1007/s10787-026-02236-z
Jian-Tong et al. (2025) High intake of n-6 polyunsaturated fatty acid exacerbates non-alcoholic steatohepatitis by the involvement of multiple metabolic pathways. Frontiers in Nutrition. 10.3389/fnut.2025.1562509
Zhiguang Huang et al. (2025) Jiawei Erzhiwan Ameliorates Androgenetic Alopecia by Regulating the SIRT1/JNK/p38 MAPK Pathway. Drug Design Development and Therapy. 10.2147/DDDT.S501823
Min Zhang et al. (2024) Matrix Gla protein suppresses osteoblast senescence and promotes osteogenic differentiation by the PI3K-AKT signaling pathway. Experimental Cell Research. 10.1016/j.yexcr.2024.114329
Öztemiz Elif et al. (2025) Regulation of transglutaminase-2 and related miRNAs expression by calorie restriction in aorta of aging female mice. Molecular Biology Reports. 10.1007/s11033-025-10892-7
Chang Xiaopan et al. (2024) Inhibition of Notch3/Hey1 ameliorates peribiliary hypoxia by preventing hypertrophic hepatic arteriopathy in biliary atresia progression. Histochemistry and Cell Biology. 10.1007/s00418-024-02278-w
Bo‑Lun Zhao et al. (2018) Effective robotic assistive pattern of treadmill training for spinal cord injury in a rat model. Experimental and Therapeutic Medicine. 10.3892/etm.2018.5822
Cai‑Yong Lai et al. (2017) Engrailed‑2 promoter hyper‑methylation is associated with its downregulation in clear cell renal cell carcinoma. Oncology Letters. 10.3892/ol.2017.7000
Akkaya H. (2021) Kisspeptin-10 Administration Regulates mTOR and AKT Activities and Oxidative Stress in Mouse Cardiac Tissue. Journal of Evolutionary Biochemistry and Physiology. 10.1134/S0022093021020095
