| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glycine--tRNA ligase;3.6.1.17 ;6.1.1.14 ;Diadenosine tetraphosphate synthetase;AP-4-A synthetase;Glycyl-tRNA synthetase;GlyRS;GARS; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GARS |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FEA-7 is a rabbit monoclonal antibody that recognizes glycine--tRNA ligase (gene GARS1) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 83.2 kDa, and the supplier reports an observed band at about 75 kDa.
Glycyl-tRNA synthetase (GARS1) attaches glycine to its tRNAs for protein synthesis and works in both the cytoplasm and mitochondria. It can also make the signaling molecule diadenosine tetraphosphate (Ap4A). Dominant GARS1 mutations cause Charcot-Marie-Tooth disease type 2D and distal spinal muscular atrophy type V.
| Target | Glycine--tRNA ligase (gene GARS1; UniProt P41250, human) |
|---|---|
| Clone | FEA-7 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 83.2 kDa |
| Observed MW | 75 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): Jurkat cells, 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human GARS.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes glycine--tRNA ligase as secreted and also located in the cytoplasm and cell projection. Tissue expression noted by UniProt (human): Widely expressed, including in brain and spinal cord... The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
