| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glycine--tRNA ligase; Diadenosine tetraphosphate synthetase; Ap4A synthetase; Glycyl-tRNA synthetase; GlyRS; Glycyl-tRNA synthetase 1; GARS1; GARS |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human GARS1 recombinant protein (Position: E61-D673). Human GARS1 shares 95.3% and 95.4% amino acid (aa) sequence identity with mouse and rat GARS1, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets glycine--tRNA ligase (gene GARS1) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA.
Glycyl-tRNA synthetase (GARS1) attaches glycine to its tRNAs for protein synthesis and works in both the cytoplasm and mitochondria. It can also make the signaling molecule diadenosine tetraphosphate (Ap4A). Dominant GARS1 mutations cause Charcot-Marie-Tooth disease type 2D and distal spinal muscular atrophy type V.
| Target | Glycine--tRNA ligase (gene GARS1; UniProt P41250, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human GARS1 fragment (Glu61–Asp673), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 95.3% and 95.4%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes glycine--tRNA ligase as secreted and also located in the cytoplasm and cell projection. Tissue expression noted by UniProt (human): Widely expressed, including in brain and spinal cord...
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
