| Field | Specification |
|---|---|
| Target | |
| Alternative names | Trifunctional purine biosynthetic protein adenosine-3; GART; PGFT; PRGS |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human GART recombinant protein (Position: E68-E1010). Human GART shares 86.2% amino acid (aa) sequence identity with mouse GART. |
| Purification | |
| Reconstitution | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects trifunctional purine biosynthetic protein adenosine-3 (gene GART) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA.
GART is a trifunctional enzyme that carries out three steps (steps 2, 3 and 5) of de novo purine synthesis leading to inosine monophosphate. Its formyltransferase activity uses a folate cofactor and is a target of antifolate drugs, so GART is studied in nucleotide metabolism and cancer.
| Target | Trifunctional purine biosynthetic protein adenosine-3 (gene GART; UniProt P22102, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human GART fragment (Glu68–Glu1010), expressed in E. coli. Sequence identity with the mouse ortholog: 86.2%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
