| Field | Specification |
|---|---|
| Target | |
| Alternative names | Trifunctional purine biosynthetic protein adenosine-3;Phosphoribosylamine--glycine ligase;6.3.4.13;Glycinamide ribonucleotide synthetase;GARS;Phosphoribosylglycinamide synthetase;Phosphoribosylformylglycinamidine cyclo-ligase;6.3.3.1;AIR synthase;AIRS;Phosphoribosyl-aminoimidazole synthetase;Phosphoribosylglycinamide formyltransferase;2.1.2.2;5'-phosphoribosylglycinamide transformylase;GAR transformylase;GART;GART;PGFT, PRGS; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GART |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FFD-7 is a rabbit monoclonal antibody that recognizes trifunctional purine biosynthetic protein adenosine-3 (gene GART) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 107.8 kDa, and the supplier reports an observed band at about 107 kDa.
GART is a trifunctional enzyme that carries out three steps (steps 2, 3 and 5) of de novo purine synthesis leading to inosine monophosphate. Its formyltransferase activity uses a folate cofactor and is a target of antifolate drugs, so GART is studied in nucleotide metabolism and cancer.
| Target | Trifunctional purine biosynthetic protein adenosine-3 (gene GART; UniProt P22102, human) |
|---|---|
| Clone | FFD-7 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 107.8 kDa |
| Observed MW | 107 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human cervix cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human GART.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
