| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Gap junction gamma-1 protein;Connexin-45;Cx45;Gap junction alpha-7 protein;GJC1;GJA7; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human GAS2, identical to the related mouse sequences. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-GAS2 Antibody Picoband® is an antibody for GAS2 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: GAS2 (Gap junction gamma-1 protein); UniProt: O43903
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 35 kDa, calculated 45470 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-GAS2 Antibody Picoband® catalog # A08589-1.
Biological background
Biological context: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Expression and localization notes: cellular localization: Cell membrane; Multi-pass membrane protein. Cell junction, gap junction., tissue context: Ubiquitously expressed..
Common research applications
- Western blotting (WB): Compare GAS2 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of GAS2 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify GAS2-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Growth arrest-specific protein 2 is a protein that in humans is encoded by the GAS2 gene. The protein encoded by this gene is a caspase-3 substrate that plays a role in regulating microfilament and cell shape changes during apoptosis. It can also modulate cell susceptibility to p53-dependent apoptosis by inhibiting calpain activity. Alternative splicing results in multiple transcript variants.
- Cross reactivity: No cross-reactivity with other proteins
- Cellular localization: Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.
- Tissue details: Ubiquitously expressed.
- Research category: Cell Adhesion,Cytoskeleton/ECM,Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
