| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Erythroid transcription factor;Eryf1;GATA-binding factor 1;GATA-1;GF-1;NF-E1 DNA-binding protein;GATA1;ERYF1, GF1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human GATA1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-GATA1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone ABCB-7; isotype Rabbit IgG; reactivity: Human. Reported application contexts include WB, IHC, ICC, IF, IP (as provided in the source record). Boster Bio Anti-GATA1 Rabbit Monoclonal Antibody catalog # M00408. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Key elements and design rationale
- Target: GATA1 (Erythroid transcription factor).
- Antibody format: Monoclonal; clone ABCB-7; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
GATA1 (protein: T-cell surface glycoprotein CD4) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed:24245781). . Reported cellular localization context: Nucleus. Tissue expression notes (as provided): Erythrocytes. .
Research relevance and current trends
- Research context keywords from the source record include: Developmental Biology,Domain Families,Epigenetics and Nuclear Signaling,Hematopoietic Progenitors,Organogenesis,Stem Cells,Transcription,Zinc Finger.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate GATA1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect GATA1 expression by Western blot in cell or tissue lysates, Detect GATA1 in FFPE tissue sections by immunohistochemistry, Localize GATA1 by immunofluorescence/immunocytochemistry in cultured cells, Enrich GATA1 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 43 kDa; calculated MW: 42751 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 43 kDa
- Cellular localization (provided): Nucleus.
- Tissue details (provided): Erythrocytes. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
