| Field | Specification |
|---|---|
| Target | |
| Alternative names | Erythroid transcription factor;Eryf1;GATA-binding factor 1;GATA-1;GF-1;NF-E1 DNA-binding protein;GATA1;ERYF1, GF1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GATA1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABCB-7 is a rabbit monoclonal antibody that recognizes erythroid transcription factor (gene GATA1) in human samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 42.8 kDa, and the supplier reports an observed band at about 43 kDa.
GATA1 is a zinc-finger transcription factor that recognizes GATA motifs and is a master regulator of red blood cell and megakaryocyte development. Working with FOG1 and other partners, it turns on globin and other erythroid genes. Mutations in GATA1 are linked to inherited anemias and thrombocytopenias and to leukemia in Down syndrome.
| Target | Erythroid transcription factor (gene GATA1; UniProt P15976, human) |
|---|---|
| Clone | ABCB-7 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 42.8 kDa |
| Observed MW | 43 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): human K562 cells, human HEL cells, human CCRF-CEM cells, human Raji cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1: 500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human GATA1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places erythroid transcription factor in the nucleus. Tissue expression noted by UniProt (human): Erythrocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
