| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Endothelial transcription factor GATA-2;GATA-binding protein 2;GATA2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human GATA2, identical to the related rat and mouse sequences. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-GATA2 Antibody Picoband® is an antibody targeting GATA2. Common applications include WB, IHC, Flow Cytometry, ELISA. Key specifications include host: Rabbit; clonality: Polyclonal; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 95 kDa; calculated MW: 50500 MW.
Boster Bio Anti-GATA2 Antibody catalog # PA1884. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Target: GATA2 — Endothelial transcription factor GATA-2
- Antibody format: Host: Rabbit; Clonality: Polyclonal; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 95 kDa; Calculated: 50500 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'.
Scientific background (datasheet): GATA2 (GATA binding protein 2) is a human gene which makes a protein called GATA binding protein 2 - a transcription factor. The GATA family of transcription factors, which contain zinc fingers in their DNA binding domain, have emerged as candidate regulators of gene expression in hematopoietic cells. Ciciotte et al. (1997)mapped the mouse Gata2 gene to chromosome 6 by study of DNA from an interspecific backcross panel. They pointed out that the human gene had been mapped to chromosome 3 by Dorfman et al. (1992) using DNA from a panel of 12 rodent/human hybrids containing various human chromosomes and applying Southern blot analysis. By exon trapping using a PAC contig spanning a breakpoint region associated with myeloid leukemia, Wieser et al. (2000) mapped the GATA2 gene to chromosome 3q21. GATA2 is transcribed from telomere to centromere. GATA2 is expressed in hematopoietic progenitors, including early erythroid cells, mast cells, and megakaryocytes, and also in nonhematopoietic embryonic stem cells.
Cellular localization (datasheet): Nucleus.
Tissue details (datasheet): Endothelial cells.
Sequence similarities (datasheet): Contains 2 GATA-type zinc fingers.
Research relevance and current trends
- Commonly studied in contexts related to Cardiogenesis,Cardiovascular,2339,Developmental Biology,Domain Families,Epigenetics and Nuclear Signaling,Lineage Markers,Lineage Specification,Stem Cells,Transcription,Transcription Factors/Regulators,Zinc Finger.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- ELISA: Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
