| Field | Specification |
|---|---|
| Target | |
| Alternative names | Trans-acting T-cell-specific transcription factor GATA-3;GATA-binding factor 3;GATA3; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GATA3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABBH-7 is a rabbit monoclonal antibody that recognizes trans-acting T-cell-specific transcription factor GATA-3 (gene GATA3) in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 47.9 kDa, and the supplier reports an observed band at about 48 kDa.
GATA3 is a zinc-finger transcription factor best known as the master regulator of T helper 2 (Th2) cell differentiation and a key factor for T-cell development. It also controls development of the kidney, inner ear and mammary gland, and it is widely used as a marker of luminal breast and urothelial carcinomas.
| Target | Trans-acting T-cell-specific transcription factor GATA-3 (gene GATA3; UniProt P23771, human) |
|---|---|
| Clone | ABBH-7 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 47.9 kDa |
| Observed MW | 48 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:2, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human thyroid cancer tissue, human small cells lung cancer tissue, rat intestine tissue, mouse pancreas tissue, human pituitary tumor tissue, human bladder cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human GATA3.
Reactivity Notes
The supplier lists reactivity with human. UniProt places trans-acting T-cell-specific transcription factor GATA-3 in the nucleus. Tissue expression noted by UniProt (human): T-cells and endothelial cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ahluwalia Amrita et al. (2020) Mechanisms by Which Membrane and Nuclear ER Alpha Inhibit Adipogenesis in Cells Isolated From Female Mice. Endocrinology. 10.1210/endocr/bqaa175
Peng Juan et al. (2019) TNF-TNFR2 Signaling Inhibits Th2 and Th17 Polarization and Alleviates Allergic Airway Inflammation. International Archives of Allergy and Immunology. 10.1159/000493583
