| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription factor GATA-4;GATA-binding factor 4;GATA4; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GATA4 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABOB-7 is a rabbit monoclonal antibody that recognizes transcription factor GATA-4 (gene GATA4) in human, mouse and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 44.6 kDa, and the supplier reports an observed band at about 54 kDa.
GATA4 is a zinc-finger transcription factor that is central to heart development and function. It works with partners such as NKX2-5 and TBX5 to activate cardiac genes and also acts in the gut, liver and gonads. Mutations in GATA4 are linked to congenital heart defects.
| Target | Transcription factor GATA-4 (gene GATA4; UniProt P43694, human) |
|---|---|
| Clone | ABOB-7 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 44.6 kDa |
| Observed MW | 54 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human HepG2 cells, human SH-SY5Y cells, rat liver tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): HepG2 cells.
Immunogen
Synthetic peptide based on the sequence of human GATA4.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transcription factor GATA-4 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Shuichang Yu et al. (2014) Differentiation of human embryonic germ cells and transplantation in rats with acute myocardial infarction. Experimental and Therapeutic Medicine. 10.3892/etm.2014.1474
