| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glutamyl-tRNA; Gln; amidotransferase subunit B, mitochondrial; Glu-AdT subunit B; Cytochrome oxidase assembly factor PET112 homolog; PET112-like; GATB; PET112; PET112L |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human GATB recombinant protein (Position: R113-E443). Human GATB shares 89.7% amino acid (aa) sequence identity with mouse GATB. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial (gene GATB) in human and mouse samples and is validated for Western blot, flow cytometry and ELISA.
GATB is the catalytic subunit of the mitochondrial glutamyl-tRNA(Gln) amidotransferase (GatCAB). Human mitochondria lack a dedicated glutaminyl-tRNA synthetase, so this complex converts glutamate that was mischarged onto tRNAGln into glutamine, allowing correct mitochondrial protein synthesis. Defects in the complex cause mitochondrial disease.
| Target | Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial (gene GATB; UniProt O75879, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human GATB fragment (Arg113–Glu443), expressed in E. coli. Sequence identity with the mouse ortholog: 89.7%.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial in the mitochondrion. Tissue expression noted by UniProt (human): Predominantly expressed in tissues characterized by high rates of oxidative phosphorylation (OxPhos), including muscle and heart.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
