| Field | Specification |
|---|---|
| Target | |
| Alternative names | GATM; AGAT; Glycine amidinotransferase, mitochondrial; EC 2.1.4.1; L-arginine:glycine amidinotransferase; Transamidinase |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human GATM recombinant protein (Position: M1-E279). Human GATM shares 95.3% and 95% amino acid (aa) sequence identity with mouse and rat GATM, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes glycine amidinotransferase, mitochondrial (gene GATM) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA.
Glycine amidinotransferase (GATM, also called AGAT) is a mitochondrial enzyme that transfers an amidino group from arginine to glycine to make guanidinoacetate. This is the rate-limiting first step of creatine synthesis, followed by methylation by GAMT. Creatine supports energy buffering in muscle, heart and brain, and GATM deficiency causes an inherited creatine deficiency.
| Target | Glycine amidinotransferase, mitochondrial (gene GATM; UniProt P50440, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human GATM fragment (Met1–Glu279), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 95.3% and 95%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Expressed in brain, heart, kidney, liver, lung, salivary gland and skeletal muscle tissue, with the highest expression in kidney.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
