| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Activator of 90 kDa heat shock protein ATPase homolog 1; AHA1; p38; AHSA1; C14orf3; HSPC322 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived mouse Gbp2 recombinant protein (Position: R181-L589). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Gbp2 Antibody Picoband® is an antibody for Gbp2 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Mouse. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: Gbp2 (activator of HSP90 ATPase activity 1); UniProt: Q9Z0E6
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 67 kDa, calculated 101272 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Gbp2 Antibody Picoband® catalog # A05737-2.
Biological background
Biological context: Acts as a co-chaperone of HSP90AA1. Activates the ATPase activity of HSP90AA1 leading to increase in its chaperone activity. Competes with the inhibitory co-chaperone FNIP1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PubMed:27353360).
Expression and localization notes: cellular localization: Cytoplasm, cytosol., tissue context: Expressed in numerous tissues, including brain, heart, skeletal muscle and kidney and, at lower levels, liver and placenta..
Common research applications
- Western blotting (WB): Compare Gbp2 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of Gbp2 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify Gbp2-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Interferon-induced guanylate-binding protein 2 is a protein that in humans is encoded by the GBP2 gene. This gene belongs to the guanine-binding protein (GBP) family, which includes interferon-induced proteins that can bind to guanine nucleotides (GMP, GDP and GTP). The encoded protein is a GTPase which hydrolyzes GTP, predominantly to GDP. The protein may play a role as a marker of squamous cell carcinomas.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cytoplasm, cytosol.
- Tissue details: Expressed in numerous tissues, including brain, heart, skeletal muscle and kidney and, at lower levels, liver and placenta.
- Research category: Chaperones,Golgi Proteins,Protein Trafficking,Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
