| Field | Specification |
|---|---|
| Target | |
| Alternative names | Guanylate-binding protein 2; GTP-binding protein 2; GBP-2; mGBP-2; mGBP2; Guanine nucleotide-binding protein 2; Interferon-induced guanylate-binding protein 2; Gbp2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Gbp2 recombinant protein (Position: R181-L589). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects guanylate-binding protein 2 (gene Gbp2) in mouse samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 66.7 kDa, and the supplier reports an observed band at about 67 kDa.
In mice, guanylate-binding protein 2 (Gbp2) is an interferon-induced GTPase that targets vacuoles containing intracellular pathogens such as Toxoplasma and Chlamydia. It helps break these vacuoles open and supports inflammasome activation, making it a common subject in studies of cell-autonomous immunity.
| Target | Guanylate-binding protein 2 (gene Gbp2; UniProt Q9Z0E6, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 66.7 kDa |
| Observed MW | 67 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): ANA-1 cells.
Immunogen
Recombinant mouse Gbp2 fragment (Arg181–Leu589), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse. UniProt places guanylate-binding protein 2 in the cytoplasmic vesicle membrane, Golgi apparatus membrane and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
