| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Voltage-dependent anion-selective channel protein 1;VDAC-1;hVDAC1;Outer mitochondrial membrane protein porin 1;Plasmalemmal porin;Porin 31HL;Porin 31HM;VDAC1;VDAC; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human GCN2 Can phosphorylate the alpha subunit of EIF2 and may mediate translational control. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-EIF2AK4 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone ACAA-5; isotype Rabbit IgG; reactivity: Human. Reported application contexts include WB, IHC, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-GCN2 EIF2AK4 Rabbit Monoclonal Antibody catalog # M01172. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: EIF2AK4 (Voltage-dependent anion-selective channel protein 1).
- Antibody format: Monoclonal; clone ACAA-5; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
EIF2AK4 (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis. . Reported cellular localization context: Mitochondrion outer membrane . Cell membrane . Tissue expression notes (as provided): Heart, liver and skeletal muscle.
Research relevance and current trends
- Research context keywords from the source record include: DNA/RNA,Epigenetics and Nuclear Signaling,Protein Phosphorylation,Ser/Thr Kinases,Signal Transduction,Translation.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate EIF2AK4 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect EIF2AK4 expression by Western blot in cell or tissue lysates, Detect EIF2AK4 in FFPE tissue sections by immunohistochemistry, Localize EIF2AK4 by immunofluorescence/immunocytochemistry in cultured cells, Quantify EIF2AK4-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 190 kDa; calculated MW: 30773 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 190 kDa
- Cellular localization (provided): Mitochondrion outer membrane . Cell membrane .
- Tissue details (provided): Heart, liver and skeletal muscle.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
